Journal
CHEMISTRY & BIOLOGY
Volume 18, Issue 3, Pages 361-371Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2011.01.011
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Funding
- National Institutes of Health [NS38486]
- NCI Cancer Center [P30 CA08748]
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We have developed a methodology for generating milligram amounts of functional Eph tyrosine kinase receptor using the protein engineering approach of expressed protein ligation. Stimulation with ligand induces efficient autophosphorylation of the semisynthetic Eph construct. The in vitro phosphorylation of key Eph tyrosine residues upon ligand-induced activation was monitored via time-resolved, quantitative phosphoproteomics, suggesting a precise and unique order of phosphorylation of the Eph tyrosines in the kinase activation process. To our knowledge, this work represents the first reported semisynthesis of a receptor tyrosine kinase and provides a potentially general method for producing single-pass membrane proteins for structural and biochemical characterization.
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