Journal
CHEMISTRY & BIOLOGY
Volume 18, Issue 3, Pages 354-360Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2011.01.010
Keywords
-
Categories
Funding
- University of Wisconsin-Madison College of Agricultural and Life Sciences
- Hans-Knoll-Institute
Ask authors/readers for more resources
Ralstonia solanacearum is a destructive crop plant pathogen and produces ralfuranone, i.e., a mono-phenyl-substituted furanone. Extensive feeding experiments with C-13-labeled L-phenylalanine now proved that all carbon atoms of the heterocycle derive, after deamination, from this aromatic amino acid. A genetic locus was identified which encodes the aminotransferase RaID and the furanone synthetase RalA. The latter is a tridomain nonribosomal peptide synthetase (NRPS)-like enzyme which was characterized (1) biochemically by the ATP-pyrophosphate exchange assay, and (2) genetically through gene inactivation and transcriptional analysis in axenic culture and in planta. This is the first study to our knowledge on the biochemical and genetic basis of R. solanacearum secondary metabolism. It implies new chemistry for NRPSs, as RaIA-mediated biosynthesis requires C-C-bond and subsequent C-O-bond formation to establish the furanone ring system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available