Journal
CHEMISTRY & BIOLOGY
Volume 18, Issue 10, Pages 1284-1289Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2011.07.017
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Funding
- EPSRC
- BBSRC
- Engineering and Physical Sciences Research Council [EP/E027660/1] Funding Source: researchfish
- EPSRC [EP/E027660/1] Funding Source: UKRI
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The ability to screen and identify new ligands for cell surface receptors has been a long-standing goal as it might allow targeting of pharmaceutically relevant receptors, such as integrins or G protein coupled receptors. Here, we present a method to amplify hits from a library of PNA-tagged peptides. To this end, human cells, overexpressing either integrins or the CCR6 receptor, were treated with a 10,000 member PNA-encoded peptide library. Extraction of the PNA tags from the surface of the cells was followed by a PNA-tag to DNA translation and amplification enabling decoding of the tags via microarray hybridization. This approach to ligand discovery facilitates screening for differences in surface-receptor ligands and/or receptor expression between different cell types, and opens up a practical approach to PNA-tag amplification.
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