Journal
CHEMISTRY & BIOLOGY
Volume 17, Issue 8, Pages 820-830Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2010.06.012
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Funding
- Deutsche Forschungsgemeinschaft [SFB/TRR34, La1289/2-1]
- Bundesministerium fur Bildung und Forschung (BMBF)
- State of Mecklenburg-Vorpom-mern [UG07068]
- Alfried Krupp von Bohlen and Halbach-Stiftung
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Little is known about intracellular metabolite pools in pathogens such as Staphylococcus aureus. We have studied a particular metabolome by means of the presented LC-MS method. By investigating the central carbon metabolism which includes most of the energy transfer molecules like nucleotides, sugar mono- and biphosphates, and cofactors, a conclusion about phenotypes and stress answers in microorganisms is possible. Quantitative metabolite levels of S. aureus grown in complex lysogeny broth and in minimal medium were compared in the wild-type S. aureus strain 8325 and the isogenic eukaryotic-like protein serine/threonine kinase (Delta pknB) and phosphatase (Delta stp) deletion mutants. Detection of several remarkable differences, e.g., in nucleotide metabolism and especially cell wall precursor metabolites, indicates a previously unreported importance of serine/threonine kinase/phosphatase on peptidoglycan and wall teichoic acid biosynthesis. These findings may lead to new insights into the regulation of staphylococcal cell wall metabolism.
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