Journal
CHEMISTRY & BIOLOGY
Volume 16, Issue 3, Pages 323-336Publisher
CELL PRESS
DOI: 10.1016/j.chembiol.2009.03.001
Keywords
-
Categories
Funding
- Searle Scholar program
- Beckman Young Investigator Program
- March of Dimes Foundation [5-FY08110]
- California Institute for Regenerative Medicine [RN1-00577-1]
- National Institutes of Health [DP2OD004744]
Ask authors/readers for more resources
Using an orthogonal tRNA-synthetase pair, unnatural amino acids can be genetically encoded with high efficiency and fidelity, and over 40 unnatural amino acids have been site-specifically incorporated into proteins in Escherichia coli, yeast, or mammalian cells. Novel chemical or physical properties embodied in these amino acids enable new means for tailored manipulation of proteins. This review summarizes the methodology and recent progress in expanding this technology to eukaryotic cells. Applications of genetically encoded unnatural amino acids are highlighted with reports on labeling and modifying proteins, probing protein structure and function, identifying and regulating protein activity, and generating proteins with new properties. Genetic incorporation of unnatural amino acids provides a powerful method for investigating a wide variety of biological processes both in vitro and in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available