Synthesis, Characterization, and DNA-Binding Studies of Mononuclear Copper(II)-Phenanthroline-Tyrosine Complex

The mononuclear complex [Cu-II(phen)((L-Tyr)(H2O)] was synthesized and structurally characterized by elemental analysis, magnetic susceptibility, UV/VIS, IR, and EPR spectroscopy, and ESI mass spectrometry. The electronic and EPR spectral data suggest a square-pyramidal geometry around Cu-II. Absorption spectra, thermal denaturation, and fluorescence-spectroscopic studies were conducted to assess the interaction of the Cu-II complex with calf thymus DNA ((7 DNA). The intrinsic binding constant for CT DNA/complex(K-b=3750M (1))and Stern-Volmer quenching constant (K=0.39) were determined.