Journal
CHEMIE INGENIEUR TECHNIK
Volume 85, Issue 1-2, Pages 95-102Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cite.201200180
Keywords
Adipose-derived stem cells; CFD model; Microcarrier; Paricle image velocimetry; Single-use spinner flask
Categories
Funding
- Swiss Commission for Technology and Innovation (CTI) [12893.1 VOUCH-LS]
Ask authors/readers for more resources
The fluid flow and suspension characteristics inside small-scale, stirred, single-use bioreactors were investigated experimentally and by means of computational fluid dynamics. The required impeller speeds for homogenous suspension were determined for two microcarrier types. The shear stress level and turbulence distribution were predicted using a numerical model, which was verified by particle image velocimetry measurements. In subsequent cultivations of primary mesencymal adipose-derived stem cells, up to 31.4-fold expansion in cell number was achieved for serum concentrations as low as 5%.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available