Journal
CHEMICAL SOCIETY REVIEWS
Volume 43, Issue 4, Pages 990-1006Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cs60245a
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Funding
- European Research Council under the European Union [291593 FLUOROCODE]
- Flemish government for the long-term structural 'Methusalem' [METH/08/04 CASAS]
- Fonds voor Wetenschapplijk Onderzoek Vlaanderen (FWO) [G.0197.11, G.0484.12]
- Hercules Foundation [HER/08/021]
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Structural and temporal inhomogeneities can have a marked influence on the performance of inorganic and biocatalytic systems alike. While these subtle variations are hardly ever accessible through bulk or ensemble averaged activity screening, insights into the molecular mechanisms underlying these diverse phenomena are absolutely critical for the development of optimized or novel catalytic systems and processes. Fortunately, state-of-the-art fluorescence microscopy methods have allowed experimental access to this intriguing world at the nanoscale. In this tutorial review we will first provide a broad overview of key concepts and developments in the application of single molecule fluorescence spectroscopy to (bio)catalysis research. In the second part topics specific to both bio and heterogeneous catalysis will be reviewed in more detail.
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