Journal
CHEMICAL SOCIETY REVIEWS
Volume 43, Issue 4, Pages 1221-1229Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cs60201j
Keywords
-
Categories
Funding
- NATIONAL CANCER INSTITUTE [F30CA174323] Funding Source: NIH RePORTER
- NCI NIH HHS [F30 CA174323] Funding Source: Medline
Ask authors/readers for more resources
Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed protein induced fluorescence enhancement'' (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available