The use of enzymes for construction of DNA-based objects and assemblies

DNA has found wide applications in DNA-based nanotechnology due to its simplicity and predictability of its secondary structure. Selecting DNA for the nanoconstruction of objects and assemblies bears the inherent potential for manipulations and control by DNA modifying enzymes. In this tutorial review, we present an overview of the enzyme-catalysed construction of DNA-based objects and assemblies. It is illustrated how a diversity of enzyme-based biochemical reactions are transferred in nanotechnological applications.