Journal
CHEMICAL RESEARCH IN TOXICOLOGY
Volume 26, Issue 2, Pages 203-212Publisher
AMER CHEMICAL SOC
DOI: 10.1021/tx3003438
Keywords
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Funding
- FEDER from Ministerio de Ciencia y Tecnologia, Spain [SAF2009-12581, AGL2009-13581-CO2-01, TRA2009-0189, AGL2010-17875]
- Xunta de Galicia, Spain [GRC 2010/10, PGDIT 07MMA006261PR, PGIDIT (INCITE) 09MMA003261PR, PGDIT (INCITE) 09261080PR, 2009/XA044, 10PXIB261254 PR]
- EU [211326-CP, 265896 BAMMBO, 265409 muAQUA, 262649 BEADS, 312184 PharmaSea]
- Atlantic Area Programme (Interreg IVB Trans-national) [2009-1/117 Pharmatlantic]
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The increasing presence of cyanotoxin producers in several regions of the world is hazardous for humans and animals. Cylindrospermopsin (CYN) is nowadays recognized as a widely distributed freshwater cyanobacterial toxin. This toxin has been shown to induce protein synthesis inhibition as well as inhibition of glutathione synthesis. Given that the liver seems to be the main target of cylindrospermopsin, in this work we used cultures of primary rat hepatocytes to study the type of cell death induced by CYN nanomolar concentrations. The involvement of reactive oxygen species in toxin induced cell death, the relationship between protein synthesis inhibition and toxicity, and the cell endogenous antioxidant response regulation were studied. We show that cylindrospermopsin induces apoptosis in primary rat hepatocytes. At the concentrations used in this work, protein synthesis inhibition and oxidative stress were involved in the cytotoxic effect elicited by the toxin. Finally, activation of the cell antioxidant response was observed at the transcriptional and translational levels.
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