Journal
CHEMICAL RESEARCH IN TOXICOLOGY
Volume 26, Issue 7, Pages 1031-1033Publisher
AMER CHEMICAL SOC
DOI: 10.1021/tx400158g
Keywords
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Funding
- National Institute of Environmental Health Sciences [R01 ES 011589]
- Natural Science and Engineering Research Council
- Canadian Institutes of Health Research
- National Center for Research Resources, National Institutes of Health [C06 RR-16572]
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The one-electron oxidation of cellular DNA in cultured human He La cells initiated by intense nanosecond 266 nm laser pulse irradiation produces cross-links between guanine and thymine bases (G*-T*), characterized by a covalent bond between C8 guanine (G*) and N3 thymine (T*) atoms. The DNA lesions were quantified by isotope dilution LC-MS/MS methods in the multiple reaction-monitoring mode using isotopically labeled [N-15, C-13]-nucleotides as internal standards. Among several known pyrimidine and 8-oxo-7,8-dihydroguanine lesions, the G*-T* cross-linked lesions were detected at levels of similar to 0.21 and 1.19 d(G*-T*) lesions per 10(6) DNA bases at laser intensities of 50 and 280 mJ/cm(2)/pulse, respectively.
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