Journal
CHEMICAL RESEARCH IN TOXICOLOGY
Volume 24, Issue 7, Pages 1003-1011Publisher
AMER CHEMICAL SOC
DOI: 10.1021/tx100420w
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Funding
- United Kingdom Department of Environment, Food and Rural Affairs [LK0984]
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There is a strong impetus to develop nonanimal based methods to predict skin sensitization potency. An approach based on physical organic chemistry, whereby chemicals are classified into reaction mechanistic domains and quantitative models or read-across methods are derived for each domain, has been the basis of several recent publications. This article is concerned with the SNAr reaction mechanistic domain. Electrophiles able to react by the SNAr mechanism have long been recognized as skin sensitizers and have been used extensively in research studies on the biology of skin sensitization. Although qualitative discriminant analysis approaches have been developed for estimating the sensitization potential for SNAr electrophiles on a yes/no qualitative basis, no quantitative mechanistic model (QMM) has so far been developed for this domain. Here, we derive a QMM that correlates skin sensitization potency, quantified by murine local lymph node assay (LLNA) EC3 data on a range of SNAr electrophiles. It is based on the Hammett sigma(-) values for the activating groups and the Taft sigma* value for the leaving group. The model takes the form pEC3 = 2.48 Sigma sigma(-) + 0.60 sigma* - 4.51. This QMM, generated from mouse LLNA data, provides a reactivity parameter 2.48 Sigma sigma(-) + 0.60 sigma*, which was applied to a set of 20 compounds for which guinea pig test results were available in the literature and was found to successfully discriminate the sensitizers from the nonsensitizers. The reactivity parameter correctly predicted a known human sensitizer 2,4-dichloropyrimidine. New LLNA data on two further SNAr electrophiles are consistent with the QMM.
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