Determination of N2-Hydroxymethyl-dG Adducts in the Nasal Epithelium and Bone Marrow of Nonhuman Primates Following 13CD2-Formaldehyde Inhalation Exposure

The presence of endogenous and exogenous N-2-hydroxymethyl-dG adducts in DNA from the nasal mucosa and bone marrow of cynomolgus macaques exposed to 1.9 and 6.1 ppm of [(CD2)-C-13]-formaldehyde for 6 h a day for 2 consecutive days was investigated using a highly sensitive nano-UPLC-MS/MS method with a limit of detection of 20 amol. Both exogenous and endogenous adducts were readily detected and quantified in the nasal tissues of both exposure groups, with an exposure dependent increase in exogenous adducts observed. In contrast, only endogenous adducts were detectable in the bone marrow, even though similar to 10 times more DNA was analyzed.