Journal
CHEMICAL PHYSICS LETTERS
Volume 495, Issue 4-6, Pages 275-279Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cplett.2010.07.005
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Funding
- European Social Fund [2009/0205/1DP/1.1.1.2.0/09/APIA/VIAA/152]
- Science and Technology Center in Ukraine [4534]
- Fundamental Research State Fund [F.28.4/007]
- Human Frontier Science Program
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The applicability of the novel fluorescent probes, aminoderivative of benzanthrone ABM, squaraine dye SQ-1 and polymethine dye V2 to identification and structural analysis of amyloid fibrils has been evaluated using the lysozyme model system in which fibrillar aggregates have been formed in concentrated ethanol solution. The association constant, binding stoichiometry and molar fluorescence of the bound dye have been determined. ABM was found to surpass classical amyloid marker ThT in the sensitivity to the presence of fibrillar aggregates. Resonance energy transfer measurements involving ABM-SQ-1 and SQ-1-V2 donor-acceptor pairs yielded the limits for fractal-like dimension of lysozyme fibrils. (C) 2010 Elsevier B.V. All rights reserved.
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