Journal
CHEMICAL PHYSICS LETTERS
Volume 480, Issue 4-6, Pages 296-299Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cplett.2009.09.005
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Funding
- MedImmune LLC ( MD, USA)
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Ethidium bromide (EB) is a commonly used probe for fluorescence detection and quanti. cation of nucleic acids, since EB forms a highly luminescent complex with dsDNA. Typical detection sensitivity of EB-based assays to dsDNA in solution is about 0.1 mu g. With the aim to increase the sensitivity of EB assays we have employed the Metal-Enhanced Fluorescence technology. We show that deposition of the EB/DNA complex solutions onto the silver nanoparticle surface additionally increases the far-field observable fluorescence approximate to 5-fold, yielding a net fluorescence enhancement factor of approximate to 180-fold, as compared to the free dye in solution. Subsequently, our approach enables the more sensitive detection of dsDNA. Published by Elsevier B.V.
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