In Vitro Culture of BMSCs on VEGF-SF-CS Three-Dimensional Scaffolds for Bone Tissue Engineering

The objective of the study was to discuss the biocompatibility of VEGF-silk fibroin-chitosan (VEGF-SF-CS) three-dimensional scaffolds in order to offer an ideal scaffold for bone tissue engineering. Bone marrow derived mesenchymal stem cells (BMSCs) were seeded on the VEGF-SF-CS three-dimensional scaffolds and silk fibroin-chitosan (SF-CS) three-dimensional scaffolds. On VEGF-SF-CS and SF-CS three-dimensional scaffolds, the cell adhesion rate was increased as time went on. Scanning electron microscopy: The cells grew actively and had normal multiple fissions, granular and filamentous substrates could be seen around the cells, and cell microfilaments were closely connected with the scaffolds. The cells could not only show attached growth on surfaces of the scaffolds, but could also extend into the scaffolds. CCK-8 and ALP analysis proved that the vascular endothelial growth factor (VEGF) could significantly promote BMSCs growth and proliferation in the SF-CS scaffolds; however, the enhancement of BMSCs cell proliferation and activity by VEGF is dependent on time. Thus, VEGF-SF-CS composite scaffolds represent a promising new type of scaffold for bone tissue engineering.