Journal
CHEMICAL PHYSICS
Volume 369, Issue 1, Pages 27-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chemphys.2010.02.004
Keywords
Roseoflavin; 8-Methylamino-riboflavin; Absorption spectroscopy; Fluorescence spectroscopy; Photo-induced charge-transfer; Photo-degradation; Triplet-singlet excitation transfer
Funding
- Deutsche Forschungsgemeinschaft (DFG) [GK640, FOR526, SFB 498]
- Cluster of Excellence
Ask authors/readers for more resources
An absorption and emission spectroscopic characterization of roseoflavin (8-dimethylamino-8-demethylriboflavin, RoF) in aqueous solutions was carried out. The studies were concentrated on roseoflavin in pH 8 phosphate buffer. Absorption cross-section spectra, fluorescence excitation spectra, fluorescence quantum distributions, fluorescence quantum yields and fluorescence lifetimes were determined. The fluorescence of RoF is quenched by photo-induced intra-molecular charge-transfer at room temperature. The photo-degradation of RoF in un-buffered water, in Tris-HCl buffer, and in phosphate buffer was studied. Phosphate buffer and to a smaller extent Tris buffer catalyse the RoF photo-degradation. Photo-excitation of the primary photoproduct, 8-methylamino-riboflavin (8-MNH-RF), enhanced the RoF degradation by triplet 8-MNH-RF - singlet RoF excitation transfer with subsequent triplet-state RoF degradation. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available