Journal
CHEMICAL GEOLOGY
Volume 504, Issue -, Pages 53-65Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chemgeo.2018.09.007
Keywords
Tree ring; alpha-Cellulose; Carbon stable isotopes (delta C-13); Oxygen stable isotopes (delta O-18); Continuous flow; High-temperature pyrolysis (HTP); Isotope ratio mass spectrometry (IRMS)
Categories
Funding
- Lamont-Doherty Earth Observatory Climate Center grant
- US National Science Foundation (NSF) [PLR-1504134, AGS-1702789, OISE-1743738, AGS 15-02150, PLR16-03473, AGS-0959148, AGS-1203818, AGS-1338734, AGS-1655188]
- BNP-Paribas Foundation
- Columbia University's Center for Climate and Life
- Fulbright-Ministerio de Ciencia e Innovacion (Spain)
- Swiss National Science Foundation [P2EZP2_152213, P300P2_164637]
- Marie-Curie FP7-PEOPLE-2012-IE grants [329935]
- [AGL-2015-73,190-JIN]
- Div Atmospheric & Geospace Sciences
- Directorate For Geosciences [1655188] Funding Source: National Science Foundation
- Swiss National Science Foundation (SNF) [P300P2_164637, P2EZP2_152213] Funding Source: Swiss National Science Foundation (SNF)
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This paper describes devices to extract a-cellulose from small whole wood samples developed at the Lamont-Doherty Earth Observatory Tree-Ring Lab and explains the procedures for chemical extractions and for the dual analysis of carbon (delta C-13) and oxygen (delta O-18) stable isotopes. Here, we provide the necessary steps and guidelines for constructing a cellulose extraction system for small amounts of wood and leaves. The system allows the simultaneous extraction of cellulose from 150 samples by means of in-house filter tubes, where chemicals used for the cellulose extraction are exchanged and eliminated in batches. This new implementation diminishes the processing time, minimizes physical sample manipulation and potential errors, increases sample throughput, and reduces the amount of chemicals and analytic costs. We also describe the dual measurement of delta C-13 and delta O-18 ratios in tree-ring cellulose using high-temperature pyrolysis in a High Temperature Conversion Elemental Analyzer (TC/EA) interfaced with a Thermo Delta V plus mass spectrometer.
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