Breaking the glass ceiling for high resolution Nd isotope records in early Cenozoic paleoceanography

We report an optimized method for extracting neodymium (Nd) from fossil fish teeth with a single-stage column (125 mu l stem volume; LN Resin, Eichrom Industries, Darien Illinois) for isotopic analysis by multi-collector inductively coupled mass spectrometry (MC-ICMPS). Three reference materials (basalt: BCR-2, BHVO-2; phosphate: fossil bone composite) and splits of fossil fish teeth samples previously processed with existing two-stage column methods were processed using the single-stage column method. Nd-143/Nd-144 values of reference materials agree within error with published values, and the values for fish teeth correspond with sample splits processed with two-stage columns. Precision to +/-similar to 0.23 epsilon(Nd) was achieved for 30 ng Nd samples of reference materials, and Nd isotope measurements of fossil fish tooth sample replicates as small as 7 ng Nd were reproducible within long term instrumental uncertainty. We demonstrate the utility of the new method with the first high resolution Nd isotope record spanning the similar to 40.0 Ma middle Eocene Climatic Optimum, which shows an excursion of 0.65 epsilon(Nd) during the peak warming at the study site (Ocean Drilling Program Leg 119, Site 738; 30 kyr sample spacing from 40.3 to 39.6 Ma). LN Resin is already used in standard methods for separating Nd, and Nd isotopes are routinely measured by MC-ICPMS with high efficiency inlet systems. Our innovation is a single, small volume LN Resin column for Nd separation. The streamlined approach results in a 10x increase in sample throughput. (C) 2009 Elsevier B.V. All rights reserved.