Journal
CHEMICAL ENGINEERING & TECHNOLOGY
Volume 31, Issue 3, Pages 463-468Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ceat.200700429
Keywords
magnetic agarose beads; optimization; protein purification
Categories
Ask authors/readers for more resources
To achieve standardized and parallelized microscale protein purification, an automated purification system was developed and optimized using a pipetting robot for purifying large sets of proteins with NI-NTA magnetic agarose beads. Recombinant hexahistidine-tagged proteins beta-glucanase (Bgl-His) and esterase I of Pseudomonas fluorescens (PFE I) were tested on the system. High purity along with high coherence was achieved. The purification procedure can be applied to 96 samples simultaneously and only takes about 1/3 of the time required for purification conducted by hand. Due to the stability of the robot system, the consistency of the results is much better than the results achieved through manual purification. This rapid and reliable method is reproducible and applicable to high throughput screening of huge amounts of samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available