4.7 Article

Multiplex detection of nucleic acids using a low cost microfluidic chip and a personal glucose meter at the point-of-care

Journal

CHEMICAL COMMUNICATIONS
Volume 50, Issue 29, Pages 3824-3826

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc00133h

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Funding

  1. National Natural Science Foundation of China [21190040, 21375034, 21175035]
  2. National Basic Research Program [2011CB911002]
  3. International Science & Technology Cooperation Program of China [2010DFB30300]
  4. Program of Young Teacher Grown of Hunan University

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A simple assay for multiplex DNA detection has been developed using a microfluidic chip and a personal glucose meter. By using this system, multiplex detection of three genotypes of hepatitis B virus DNA was possible with a detection limit of 10 pM. This point-of-care assay represented a versatile platform for sensitive multiplex target detection.

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