Journal
CHEMICAL COMMUNICATIONS
Volume 50, Issue 36, Pages 4733-4735Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc00256c
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Funding
- National Natural Science Foundation of China [21325523]
- Award for the Hundred Talent Program of the Chinese Academy of Sciences
- Fund for Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development [(2012) 433]
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We develop a new method for the sensitive detection of polynucleotide kinase (PNK) using rolling circle amplification-induced chemiluminescence. This method exhibits high sensitivity with a detection limit of 2.20 x 10(-4) U mL(-1), which is superior to most reported approaches. Moreover, this method can be used to screen both the inhibitors and the activators of PNK, and can be further applied for real sample analysis.
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