Journal
CHEMICAL COMMUNICATIONS
Volume 50, Issue 50, Pages 6578-6581Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc48460b
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Funding
- National Basic Research 973 Program [2014CB932004]
- National Natural Science Foundation of China [21035006, 21275120]
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A novel element-tagged activity-based photo-cleavable biotinylated chemical ``Hub'' was designed and synthesized to orthogonally integrate ICP-MS and ESI-MS for absolute targeted-protein quantification. This tetrafunctional chemical ``hub'' allowed us to quantify a targeted protein using species-unspecific isotope dilution ICP-MS and to know which protein is being quantified using ESI-IT-MS at the same time.
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