4.7 Article

ELISA detection of semicarbazide based on a fast sample pretreatment method

Journal

CHEMICAL COMMUNICATIONS
Volume 49, Issue 55, Pages 6164-6166

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc42790k

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Funding

  1. Ministry of Science and Technology 973 Project [2013CB967100]

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A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 mu g L-1 of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.

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