Journal
CHEMICAL COMMUNICATIONS
Volume 49, Issue 37, Pages 3869-3871Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc00203a
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Funding
- National Natural Science Funds of China [21005061]
- Specialised Research Fund for the Doctoral Programme of Higher Education of China [20096101120011]
- Natural Science Basic Research Plan in Shaanxi Province of China [2010JQ2013, 2012KJXX-25]
- Natural Science Foundation of Northwest University
- NWU Graduate Cross-Discipline Funds [10YJC19]
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A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 x 10(-6) U mL(-1), using a supersandwich amplification technique.
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