Journal
CHEMICAL COMMUNICATIONS
Volume 49, Issue 47, Pages 5363-5365Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc42308e
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Funding
- ERC [FCC 266804]
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A ratiometric Eu-III complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pK(a) of 7.15.
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