Journal
CHEMICAL COMMUNICATIONS
Volume 49, Issue 77, Pages 8599-8601Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc42543f
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Funding
- Legacy Heritage fund program of the Israel Science Foundation
- Marie Curie Career Integration Grant
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Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.
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