4.7 Article

A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization

Journal

CHEMICAL COMMUNICATIONS
Volume 49, Issue 74, Pages 8247-8249

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc44125c

Keywords

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Funding

  1. National Natural Science Foundation of China [21205040, 21075040, 31101285]
  2. Shanghai Fund [12ZR1442700, 11nm0502500]
  3. Fundamental Research Funds for the Central Universities
  4. Hitachi, Ltd.

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Via the base-stacking hybridization strategy, we have developed a universal, one-step real-time quantitative PCR assay for sensitive and selective detection of microRNAs. This proposed assay has several intrinsic features including rapid response, low cost, simple handling procedures, etc.

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