4.7 Article

Universal labeling of 5′-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity

Journal

CHEMICAL COMMUNICATIONS
Volume 49, Issue 66, Pages 7322-7324

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc44454f

Keywords

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Funding

  1. US National Aeronautics and Space Administration (NASA) through the NASA Astrobiology Institute-Ames Research Center [NNX09AH70A]
  2. US National Institutes of Health (NIH) [T32 GM08347]
  3. Biocatalysis Initiative of the BioTechnology Institute at the University of Minnesota
  4. NASA [117502, NNX09AH70A] Funding Source: Federal RePORTER

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An artificial RNA ligase specific to RNA with a 5'-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing.

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