4.7 Article

Aligning an endoglucanase Cel5A from Thermobifida fusca on a DNA scaffold: potent design of an artificial cellulosome

Journal

CHEMICAL COMMUNICATIONS
Volume 49, Issue 62, Pages 6971-6973

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc42614a

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Funding

  1. Advanced Low Carbon Technology Research and Development Program (ALCA) from the Japan Science and Technology Agency (JST)
  2. Japan Society for the Promotion of Science (JSPS) for Young Scientists
  3. Grants-in-Aid for Scientific Research [12J06340] Funding Source: KAKEN

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A novel multi-cellulase conjugate assembled on a double-stranded DNA scaffold, a DNA-(endoglucanase)(n) conjugate, exhibited unique hydrolytic activity toward crystalline cellulose (Avicel) depending on the cellulase/DNA ratio on the DNA-based artificial cellulosome.

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