Journal
CHEMICAL COMMUNICATIONS
Volume 49, Issue 63, Pages 7061-7063Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc43619e
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Funding
- Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA)
- Centre National de la Recherche Scientifique (CNRS)
- Agence Nationale de la Recherche [ANR-08-PANH-008]
- Spanish MINECO [CTQ2012-32448]
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This study shows how the NiFeSe site of an anaerobically purified O-2-resistant hydrogenase reacts with air to give a seleninate as the first product. Less oxidized states of the active site are readily reduced in the presence of X-rays. Reductive enzyme activation requires an efficient pathway for water escape.
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