Journal
BIOCONJUGATE CHEMISTRY
Volume 11, Issue 2, Pages 289-291Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc990152n
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM057356] Funding Source: NIH RePORTER
- NIGMS NIH HHS [GM 57356] Funding Source: Medline
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A new anchoring group is described for binding oligonucleotides to gold surfaces. On the basis of a ketal derived from 4,5-dihydroxy-1,2 dithiane and epiandrosterone, it is easy to prepare and to link to oligonucleotides. Gold nanoparticle-oligonucleotide conjugates made using this cyclic disulfide linker serve as effective probes far detecting specific oligonucleotide sequences, and they exhibit much greater stability toward dithiothreitol than corresponding conjugates prepared with the conventional mercaptohexyl group or an acyclic disulfide unit. The high stability toward thiol deactivation likely results, in part at least, from anchoring each oligonucleotide to gold through two sulfur atoms.
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