Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 80, Pages 10031-10033Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc35648a
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Funding
- National Basic Research Program of China (973 Program) [2012CB720600, 2012CB720603, 2012CB720605]
- National Science of Foundation of China [90813031, 30973605, 21102108]
- National Grand Program on Key Infectious Disease [2012ZX10003002-014]
- State Key Laboratory of Bioorganic
- Natural Products Chemistry, Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1030]
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The analysis of DNA methylation and MTase (methyltransferase) activity is important in epigenetic study. We have developed a novel strategy for sensitive analysis of MTase activity based on a hairpin shaped DNAzyme; 8-17 DNAzyme amplicon has been adopted and found to be very effective in such analysis.
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