Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 4, Pages 498-500Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc15659d
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- Department of Science and Technology, India
- CSIR, India
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Direct incorporation of azide groups into RNA oligonucleotides by in vitro transcription reactions in the presence of a new azide-modified UTP analogue, and subsequent posttranscriptional chemical labeling of azide-modified oligoribonucleotide transcripts by click and Staudinger reactions are described. This postsynthetic labeling protocol is robust and modular, and offers an alternative access to RNA labeled with biophysical probes.
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