Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 42, Pages 5127-5129Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc30373f
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Funding
- ARIN S.p.A. - Naples (Italy)
- FIRB by MIUR [RBPR05JH2P]
- [NIH-P41-RR003155]
- [NIH-P50-GM076516]
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A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy.
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