Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 11, Issue 6, Pages 657-673Publisher
VSP BV
DOI: 10.1163/156856200743931
Keywords
chicken antibodies; protein adsorption; latex particles; particle-enhanced immunoassays.
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The use of egg yolk antibodies (IgY) instead of Ige from mammalian species may present several advantages in the development of routine diagnostic immunoassays, On the one hand, the animal suffering is reduced, as antibodies are obtained directly from the egg. On the other hand, the use of IyY avoids the rheumatoid factor interference. The rheumatoid factor interacts with Ige molecules in many immunoassays causing false positive results. Despite these advantages, IgY antibodies are scarcely used. As part of an aim to develop a diagnostic test based on IgY-latex agglutination, a preliminary study on some characteristics of the IgY-latrx complexes is carried out. In this work, protein adsorption and desorption, isoelectric point, electrokinetic mobility, and colloidal stability are analysed. Results are compared to those obtained by IgC. Interesting differences are observed (which mainly arise from the difference in molecular structure between TgY and IgG), suggesting that IgY is a more hydrophohic molecule than IgG. In addition, colloidal dispersions; of IgY-covered Inter particles are more stable (at pn 8) than those sensitized by IgG.
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