Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 72, Pages 9059-9061Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc34379g
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Funding
- National Science Foundation [CHE0746446]
- American Heart Association [11PRE7890040]
- Camille Dreyfus Foundation
- Maryland Biotechnology Award
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0746446] Funding Source: National Science Foundation
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C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.
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