Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 13, Pages 1898-1900Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc16156g
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The performance of immobilized enzyme systems is often limited by cofactor diffusion and regeneration. Here, we demonstrate an engineered enzyme capable of utilizing the minimal cofactor nicotinamide mononucleotide (NMN+) to address these limitations. Significant gains in performance are observed with NMN+ in immobilized systems, despite a decreased turnover rate with the minimal cofactor.
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