Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 53, Pages 6681-6683Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc31662e
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Funding
- A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council [10/1/21/19/656]
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We report the first fluorescent diamino-chalcone library and its application in the discovery of a mouse embryonic stem cell (mESC) probe. CDg4, a novel green fluorescent mESC probe was discovered through a high-content image based screening of 160 members of the chalcone library. Interestingly, the molecular binding target of CDg4 was identified as the glycogen of the stem cell colony surface, rather than a conventional protein target from an intracellular source.
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