4.7 Article

Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells

Journal

CHEMICAL COMMUNICATIONS
Volume 48, Issue 68, Pages 8595-8597

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc33565d

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Funding

  1. Estonian Ministry of Education and Sciences [SF0180121s08]
  2. Estonian Science Foundation [8230, 8419]
  3. U.S. National Institutes of Health [R01GM081030]

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A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy.

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