4.7 Article

Comparative proteomic profiling of mammalian cell lysates using phosphopeptide microarrays

Journal

CHEMICAL COMMUNICATIONS
Volume 48, Issue 16, Pages 2240-2242

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc17701c

Keywords

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Funding

  1. Ministry of Education [R-143-000-394-112]
  2. Agency for Science, Technology and Research [R-143-000-391-305]
  3. DSO National Laboratories

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A library of 176 human phosphotyrosine-containing peptides was used to establish cell lysate binding profiles in a two colour microarray format. The resulting hits led to the pull-down and identification of biomarkers associated with cancer states.

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