Journal
PROTOPLASMA
Volume 213, Issue 3-4, Pages 184-193Publisher
SPRINGER-VERLAG WIEN
DOI: 10.1007/BF01282156
Keywords
actin; calyculin A; phosphatases; transvacuolar strand; cytoplasmic streaming; root hair cell
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In root hair cells of Limnobium stoloniferum, a protein phospha;ase inhibitor, calyculin A (CA), at concentrations higher than 50 nM inhibits cytoplasmic streaming and induces remarkable morphological changes in the cytoplasm: the transvacuolar strands disperse and spherical cytoplasmic bodies emerge. The mechanism of the morphological changes of the cytoplasm induced by CA was studied by pharmacological analyses. The formation of spherical bodies in cells treated with CA was suppressed by the actin-depolymerizing and -fragmenting drugs latrunculin B and cytochalasin D at concentrations higher than 100 nM and 5 mu M, respectively. In contrast, 100 mu M propyzamide, a microtubule-depolymerizing drug, did not affect the formation of spherical bodies by CA. Interestingly, 60 mM 2,3-butanedione monoxime, an inhibitor of myosin, also suppressed the CA-induced formation of cytoplasmic spherical bodies. These results indicate that the actin cytoskeleton is intimately involved in the morphological changes of the cytoplasm induced by CA.
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