Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 51, Pages 6393-6395Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc32521g
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Funding
- Morvus Technologies
- BBSRC
- University of Nottingham
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Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.
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