4.7 Article

Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit

Journal

CHEMICAL COMMUNICATIONS
Volume 48, Issue 51, Pages 6360-6362

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc32038j

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Funding

  1. Research Grants Council of Hong Kong [HKUST2/CRF/10, N_HKUST620/11]

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A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC-dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.

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