Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 67, Pages 8419-8421Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc31887c
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Funding
- BBSRC grant [BB/H003746/1]
- EPSRC grant [EP/H045848/1]
- BBSRC [BB/E007384/1, BB/H003746/1] Funding Source: UKRI
- EPSRC [EP/H045848/1, EP/J015318/1] Funding Source: UKRI
- MRC [MC_G0900868] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H003746/1, BB/E007384/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/H045848/1, EP/J015318/1] Funding Source: researchfish
- Medical Research Council [MC_G0900868] Funding Source: researchfish
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GFP and a FRET compatible dye were used to assess the influence of genetically encoded aryl azide positioning on Click chemistry-based protein conjugation. While modification efficiency of the sampled mutants using a strain promoted reaction varied by as much as similar to 10 fold, there was no simple correlation with accessibility of the aryl azide on GFP's surface. One labeled GFP mutant (Gln204AzPhe) exhibited high efficiency FRET (similar to 90%) and an unprecedented pseudo-Stokes shift of 126 nm.
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