Amplification of G-quadruplex DNAzymes using PCR-like temperature cycles for specific nucleic acid and single nucleotide polymorphism detection

A nucleic acid sensor, based on the amplified formation of G-quadruplex DNAzymes by polymerase chain reaction (PCR)-like temperature cycles, was developed. This turn-on'' process allowed effective detection of specific nucleic acid targets and identification of single nucleotide polymorphisms (SNPs).