Journal
CHEMICAL COMMUNICATIONS
Volume 47, Issue 46, Pages 12536-12537Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc15476a
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Funding
- Deutsche Forschungsgemeinschaft [Ja 794-3]
- Volkswagen foundation [VW-I/82549]
- Fonds der Chemischen Industrie
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We report the first example of RNA labeling based on inverse electron-demand Diels-Alder reactions. Both chemically synthesized and enzymatically transcribed RNAs were successfully modified with biotin or a fluorescent label. This approach works efficiently under mild conditions in water and does not require transition metals.
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