Journal
CHEMICAL COMMUNICATIONS
Volume 47, Issue 16, Pages 4763-4765Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc10325c
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Funding
- National Natural Science Foundations of China [20875019]
- National Basic Research Program of China [2010CB534911]
- Natural Science Foundations of Guangxi Province [0991012Z, 2010GXNSFF013001]
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We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0 x 10(-4) U mL(-1) with a detection range from 5.0 x 10(-4) to 10 U mL(-1)
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