Journal
STEM CELLS
Volume 18, Issue 3, Pages 190-195Publisher
ALPHAMED PRESS
DOI: 10.1634/stemcells.18-3-190
Keywords
megakaryocyte progenitors; cord blood; hematopoietic cell transplantation
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Delayed platelet recovery is an inherent problem with cord blood cell transplantation (CBCT), To investigate this problem, the number of human megakaryocyte (MK) progenitor cells in cord blood (CB; n = 24) was measured and compared with that in G-CSF-mobilized peripheral blood stem cells (PBSC; n = 25), The median numbers of colony-forming units for MK (CFU-MK) that were detected by a sec-free assay system in CB and peripheral blood (PB) were 26 (range, 6-102)/10(5) nucleated cells (NC) and 37 (2-540)/10(5) mononuclear cells (MNC), respectively. The numbers of colony-forming units for granulocyte/macrophage (CFU-GM) were 88 (33-241)/10(5) NC in CB and 138 (6.3-1,250)/10(5 M)NC in PB, The frequencies of CD34(+) cells in CB and PB were, respectively, 0.44% (0.10-1.07) and 0.98% (0.05-20.8). The numbers of CFU-MK in CB and PBSC were correlated with those of CD34(+) cells. The estimated number of infused CFU-MK in CBCT was 1/15 that of PBSC transplantation (PBSCT), based upon the above data and the widely used standard doses for both types of transplants. Further, the numbers of infused CFU-MK in patients who received allogeneic PBSCT at our institute were inversely correlated with the speed of platelet recovery, These data indicate that delayed platelet recovery after CBCT is simply due to the low number of CFU-MK contained in grafts.
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